Oh 2014, ARF6 ChIP-seq.
ChIP-seq analysis of the ARF6 transcription factor. ARFs are
transcription factors that bind the DNA sequence 5'-TGTCTC-3'
found in the auxin-responsive promoter elements
(AuxREs). Transgenic plants expressiong ARF6-Myc driven by
native ARF6 promoter (ARF6p::ARF6-Myc) are grown in the dark
for 5 days. The etiolated seedlings are then used as input for
chromatin extraction with an anti-myc antibody.
- Raw data was downloaded
- Input file format: SRA
- Download date: n/a
From A. thaliana (Feb 2011 TAIR10/araTha1).
FASTQ files were extracted from SRA files using fastq-dump
toolkit v2.5.0) and mapped to the genome
v0.12.8. SAM files were then converted into bam
v0.1.14 and to bed using bamToBed v2.12.0
conversion was carried out using bed2sga.pl
Oh E, Zhu JY, Bai MY, Arenhart RA, Sun Y, Wang ZY
Cell elongation is regulated through a central circuit of interacting transcription factors in the Arabidopsis hypocotyl.
Elife. 2014 May 27;3. doi: 10.7554/eLife.03031.
Interactions of ARF6 with PIF4, BZR1, and RGA integrate auxin
signaling with environmental and other hormonal signals in